Determination of triphenyltin and its metabolite diphenyltin in culture medium by high-performance liquid chromatography with UV detection

H. T. LU, H. B. LI, F. CHEN, Ming Hung WONG, Y. JIANG

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5 Citations (Scopus)

Abstract

A method for the determination of triphenyltin and diphenyltin was developed by reversed-phase high-performance liquid chromatography with UV detection. Triphenyltin and diphenyltin were separated using a reversed-phase Symmetry C₁₈ column (150 × 3.9 mm, 5 μm) with tetrahydrofuran-water-acetonitrile-glacial acetic acid (13:25:5:7, v/v) containing 0.05% triethylamine and 1.0% sodium acetate as mobile phase at 0.50 mL min⁻¹ and detection at 257 nm. The calibration curves were linear from 0.26 μmol L⁻¹ to 1100 μmol L⁻¹ for triphenyltin with a correlation coefficient of 0.9999 (n=12) and from 0.60 μmol L⁻¹ to 1200 μmol L⁻¹ for diphenyltin with a correlation coefficient of 0.9991 (n=12), respectively. The detection limits of triphenyltin and diphenyltin were 0.2 μmol L⁻¹ and 0.4 μmol L⁻¹, respectively. The method was successfully applied to the determination of triphenyltin and its metabolite diphenyltin in culture medium. The recoveries of triphenyltin and diphenyltin were in the ranges of 97.7% to 103.3% and 85.5% to 91.6%, respectively. Copyright © 2004 Friedr. Vieweg & Sohn Verlagsgesellschaft mbH.
Original languageEnglish
Pages (from-to)113-116
JournalChromatographia
Volume60
Issue number1-2
Publication statusPublished - Jul 2004

Citation

Lu, H. T., Li, H. B., Chen, F., Wong, M. H., & Jiang, Y. (2004). Determination of triphenyltin and its metabolite diphenyltin in culture medium by high-performance liquid chromatography with UV detection. Chromatographia, 60(1-2), 113-116.

Keywords

  • Column liquid chromatography
  • Triphenyltin
  • Diphenyltin
  • Culture medium

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