Design and synthesis of heterobimetallic donor-acceptor chemodosimetric ensembles for the detection of sulfhydryl-containing amino acids and peptides

Cheuk Fai Stephen CHOW, Michael H. W. LAM, Hongyan SUI, Wai-Yeung WONG

Research output: Contribution to journalArticles

21 Citations (Scopus)

Abstract

A competitive indicator displacement assay has been successfully developed for the ratiometric determination of sulfhydryl-containing amino acids and peptides using heterobimetallic donor-acceptor complexes as chemodosimetric ensembles. Chromotropic cis-[ML2(CN)2] (M = Fe II, RuII, OsII; L = diimine) are used as signaling indicators and PtII(DMSO)Cl2 acceptor moiety is used as the receptor for the sulfhydryl-containing analytes. A series of three heterobimetallic donor-acceptor complexes: cis-FeII(bpy) 2[CN-PtII(DMSO)Cl2]2 (1), cir-RuII(bpy)2[CN-PtII(DMSO)Cl 2]2 (2) and cis-OsII(bpy)2[CN- PtII(DMSO)Cl2]2 (3) are synthesized and characterized by X-ray crystallography. All the three ensembles are able to produce specific colorimetric/fluorimetric responses to sulfhydryl-containing amino acids (cysteine, homocysteine and methionine) as well as the sulfhydryl-containing small peptide glutathione. The mechanism of the competitive displacement assay is evaluated by examining the thermodynamics of formation of the donor-acceptor linkage and adducts between the acceptor metal and the sulfhydryl-containing analytes as well as by systematic variation of the donor and acceptor metals in the chemodosimetric ensembles. Copyright © 2005 Royal Society of Chemistry.

Original languageEnglish
Pages (from-to)475-484
JournalDalton Transactions
Volume2005
Issue number3
DOIs
Publication statusPublished - 2005

Citation

Chow, C.-F., Lam, M. H. W., Sui, H., & Wong, W.-Y. (2005). Design and synthesis of heterobimetallic donor-acceptor chemodosimetric ensembles for the detection of sulfhydryl-containing amino acids and peptides. Dalton Transactions, 2005(3), 475-484. doi: 10.1039/B410974K

Fingerprint Dive into the research topics of 'Design and synthesis of heterobimetallic donor-acceptor chemodosimetric ensembles for the detection of sulfhydryl-containing amino acids and peptides'. Together they form a unique fingerprint.