Cultured gill epithelial cells from tilapia (Oreochromis niloticus): A new in vitro assay for toxicants

Bingsheng ZHOU, Wenhua LIU, Shiu Sun Rudolf WU, Paul K. S. LAM

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A culture gill epithelium from seawater-adapted tilapia (Oreochromis niloticus) was developed for testing PAHs and dioxin-like contaminants in seawater. The epithelia consists two to three layers of epithelial cells incorporating both pavement cells and mitochondria-rich cells (MRCs). Polarity and a stable transepithelial resistance (TER) were maintained, and closely resembled those in fish gills in vivo. The tightness (integrity) of the epithelia remained unchanged upon exposure to benzo[a]pyrene (B[a]P), 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and 3,3′,4,4′,5- pentachlorobiphenyl (PCB#126), while a concentration-dependent response of EROD activity in the epithelia was induced within 18-24 h when the apical side was exposed to these toxicants. The 24 h EC 50 of EROD activity was 2.77 × 10 -7 M for PCB#126, 1.85 × 10 -7 M for B[a]P and 7.38 × 10 -10 M for TCDD, showing that the preparation was not only sensitive to PAHs and dioxin-like compounds, but also able to produce inductive potency of AhR agonists that generally agreed with those derived from other established in vitro and in vivo systems. The results suggest that the cultured gill epithelia from seawater-adapted tilapia may serve as a simple, rapid and cost-effective tool for assessing exposure and potential effects of toxicants in marine waters. Copyright © 2004 Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)61-72
JournalAquatic Toxicology
Volume71
Issue number1
DOIs
Publication statusPublished - 18 Jan 2005

Fingerprint

Tilapia (Cichlidae)
Tilapia
Cichlids
Oreochromis niloticus
toxic substances
3,4,5,3',4'-pentachlorobiphenyl
dioxin
gills
epithelial cells
epithelium
Epithelium
Epithelial Cells
Seawater
tetrachlorodibenzo-p-dioxin
assays
seawater
pyrene
Cytochrome P-450 CYP1A1
Dioxins
dioxins

Citation

Zhou, B., Liu, W., Wu, R. S. S., & Lam, P. K. S. (2005). Cultured gill epithelial cells from tilapia (Oreochromis niloticus): A new in vitro assay for toxicants. Aquatic Toxicology, 71(1), 61-72. doi: 10.1016/j.aquatox.2004.10.013

Keywords

  • Gill epithelia
  • Primary cell culture
  • EROD
  • Tilapia
  • B[a]P
  • PCB
  • TCDD