Continuous 17α-ethinylestradiol exposure impairs the sperm quality of marine medaka (Oryzias melastigma)

Xian QIN, Keng Po LAI, Shiu Sun Rudolf WU, Richard Yuen Chong KONG

Research output: Contribution to journalArticlespeer-review

Abstract

17α-ethinylestradiol (EE2) is an anthropogenic estrogen that is widely used for hormone therapy and oral contraceptives. It was reported that EE2 exposure induced reproductive impairments through processes affecting reproduction behavior and inducing ovotestis. However, the effects of continuous EE2 exposure on the reproductive performance remain largely unknown. In this study, adult marine medaka fish (Oryzias melastigma) were exposed to EE2 (85 ng/L) for one (F₀) and two (F₁) generations. Our results indicate that continuous EE2 exposure reduced fecundity and sperm motility. The testicular transcriptome, followed by bioinformatic analysis revealed the dysregulation of pathways related to steroidogenesis, sperm motility, and reproductive system development. Collectively, our findings indicate that continuous EE2 exposure directly affected sperm quality via the alteration of steroidogenesis and dysregulation of reproductive system development. The identified key factors including DNM1, PINK1, PDE7B, and SLC12A7 can serve as biomarkers to assess EE2-reduced sperm motility. Copyright © 2022 Elsevier Ltd. All rights reserved.

Original languageEnglish
Article number114093
JournalMarine Pollution Bulletin
Volume183
Early online dateSep 2022
DOIs
Publication statusPublished - Oct 2022

Citation

Qin, X., Lai, K. P., Wu, R. S. S., & Kong, R. Y. C. (2022). Continuous 17α-ethinylestradiol exposure impairs the sperm quality of marine medaka (Oryzias melastigma). Marine Pollution Bulletin, 183. Retrieved from https://doi.org/10.1016/j.marpolbul.2022.114093

Keywords

  • Ethinylestradiol
  • Fish
  • Reproduction
  • Multigeneration
  • Sperm quality
  • Biomarkers

Fingerprint

Dive into the research topics of 'Continuous 17α-ethinylestradiol exposure impairs the sperm quality of marine medaka (Oryzias melastigma)'. Together they form a unique fingerprint.