Complete degradation of the endocrine disruptor di-(2-ethylhexyl) phthalate by a novel Agromyces sp. MT-O strain and its application to bioremediation of contaminated soil

Hai-Ming ZHAO, Huan DU, Jing LIN, Xue Bin CHEN, Yan-Wen LI, Hui LI, Quan-Ying CAI, Ce-Hui MO, Hua-Ming QIN, Ming Hung WONG

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28 Citations (Scopus)

Abstract

A newly isolated strain Agromyces sp. MT-O could utilize various phthalates and efficiently degraded di-(2-ethylhexyl) phthalate (DEHP). Response surface methodology was successfully employed for the optimization of culture conditions including pH (7.2), temperature (29.6), and inoculum size (OD₆₀₀ of 0.2), resulting in almost complete degradation of DEHP (200 mg L¯¹) within 7 days. At different initial concentrations (50–1000 mg L¯¹), DEHP degradation curves were fitted well with the first-order kinetic model, and the half-life of DEHP degradation ranged from 0.83 to 2.92 days. Meanwhile, the substrate inhibition model was used to describe the special degradation rate with qₘₐₓ, Kₛ, and Kᵢ of 0.6298 day¯¹, 86.78 mg L¯¹, and 714.3 mg L¯¹, respectively. The GC–MS analysis indicated that DEHP was degraded into mono-ethylhexyl phthalate and phthalate acid before its complete mineralization. Bioaugmentation of DEHP-contaminated soils with strain MT-O has greatly enhanced DEHP disappearance rate in soils, providing great potential for efficiently remediating DEHP-contaminated environment. Copyright © 2016 Published by Elsevier B.V.
Original languageEnglish
Pages (from-to)170-178
JournalScience of the Total Environment
Volume562
DOIs
Publication statusPublished - 15 Aug 2016

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endocrine disruptor
phthalate
bioremediation
degradation
contaminated soil
half life
mineralization

Citation

Zhao, H.-M., Du, H., Lin, J., Chen, X.-B., Li, Y.-W., Li, H. ... Wong, M. H. (2016). Complete degradation of the endocrine disruptor di-(2-ethylhexyl) phthalate by a novel Agromyces sp. MT-O strain and its application to bioremediation of contaminated soil. Science of the Total Environment, 562, 170-178. doi: 10.1016/j.scitotenv.2016.03.171.

Keywords

  • Di-(2-ethylhexyl) phthalate
  • Agromyces sp
  • Biodegradation
  • Response surface methodology
  • Degradation pathway
  • Soil bioremediation