Cadmium hyperaccumulation leads to an increase of glutathione rather than phytochelatins in the cadmium hyperaccumulator Sedum alfredii

Qin SUN, Zhi Hong YE, Xiao Rong WANG, Ming Hung WONG

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125 Citations (Scopus)

Abstract

Sedum alfredii has been reported to be a cadmium (Cd) hyperaccumulator. Phytochelatins (PCs) and other thiol (SH)-containing compounds have been proposed to play an important role in the detoxification and tolerance of some heavy metals, but it is not clear whether PCs are responsible for Cd hyperaccumulation and tolerance in S. alfredii. In this study, two geographically isolated populations of S. alfredii were studied: one population grew on an old Pb/Zn mine site, while the other on a non-mine site. The mine population of this species exhibited a stronger heavy metal tolerance than in the other population. Root-to-shoot transport of Cd was higher in population located at the mine site than at the non-mine site. Considerable amounts of Cd were accumulated in leaves and stems of mine plants, while most Cd was distributed in roots of non-mine plants. Non-protein SH in plant tissues of two populations were further investigated by a HPLC pre-column derivatization system. Upon exposure to Cd, no PCs were detected in all tissues of mine population, while an appreciable amount of glutathione (GSH) was observed in the descending order of stem>root>leaf. The concentrations of GSH consistently increased with the increase of exogenous Cd concentrations and time. On the contrary, Cd exposure strongly induced the production of PCs (mainly PC₂ and PC₃) and GSH in plant tissues of non-mine population, and the concentrations of GSH showed an initial drop over the duration of 7-d exposure. The present results provided strong evidence that PCs are not involved in Cd transport, hyperaccumulation and tolerance in mine population of S. alfredii. Copyright © 2007 Elsevier GmbH. All rights reserved.

Original languageEnglish
Pages (from-to)1489-1498
JournalJournal of Plant Physiology
Volume164
Issue number11
DOIs
Publication statusPublished - 09 Nov 2007

Fingerprint

Sedum
Phytochelatins
phytochelatins
hyperaccumulators
Cadmium
Glutathione
glutathione
cadmium
Population
metal tolerance
Heavy Metals
plant tissues
Plant Stems
stems
derivatization
thiols
Sulfhydryl Compounds
leaves
High Pressure Liquid Chromatography

Citation

Sun, Q., Ye, Z. H., Wang, X. R., & Wong, M. H. (2007). Cadmium hyperaccumulation leads to an increase of glutathione rather than phytochelatins in the cadmium hyperaccumulator Sedum alfredii. Journal of Plant Physiology, 164(11), 1489-1498. doi: 10.1016/j.jplph.2006.10.001

Keywords

  • Cadmium hyperaccumulator
  • Glutathione
  • Heavy metal tolerance
  • Phytochelatins
  • Sedum alfredii