Biodegradation pathway of di-(2-ethylhexyl) phthalate by a novel Rhodococcus pyridinivorans XB and its bioaugmentation for remediation of DEHP contaminated soil

Hai-Ming ZHAO, Rui-Wen HU, Xue-Xue CHEN, Xue-Bin CHEN, Huixiong LÜ, Yan-Wen LI, Hui LI, Ce-Hui MO, Quan-Ying CAI, Ming Hung WONG

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14 Citations (Scopus)

Abstract

A novel bacterial strain designated as Rhodococcus pyridinivorans XB, capable of utilizing various endocrine disruptor phthalates or phthalic acid (PA) as sole source of carbon and energy, was isolated from activated sludge. Under the optimal culture conditions (pH 7.08, 30.4 °C, inoculum size (OD600 nm) of 0.6) obtained by response surface methodology, di-(2-ethylhexyl) phthalate (DEHP, 200 mg/L) could be degraded by strain XB with a removal rate of 98% within 48 h. Under the observation of an atomic force microscope, it was confirmed that DEHP did not inhibit the growth of strain XB which might produce some extracellular polymeric substances as a response to DEHP stress, resulting in rapid degradation of DEHP. At initial concentrations of 50–800 mg/L DEHP, its degradation curves were well fitted with the first-order kinetic model, and the half-life of DEHP degradation varied from 5.44 to 23.5 h. The degradation intermediates of DEHP were identified by both GC–MS and high performance liquid chromatography–time of flight-mass spectrometry (HPLC–TOF-MS). Significant up-regulation was observed for the relative expression levels of genes (i.e., phthalate hydrolase, PA 3,4-dioxygenase, protocatechuate 3,4-α and 3,4-β dioxygenase) involved in DEHP degradation determined by real-time quantitative PCR (RT-qPCR). A DEHP biodegradation pathway by strain XB was proposed based on the identified intermediates and the degrading genes. Bioaugmentation of DEHP-contaminated soils with strain XB could efficiently promote DEHP removal, offering great potential in bioremediation of DEHP-contaminated environment. Copyright © 2018 Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)1121-1131
JournalScience of the Total Environment
Volume640-641
Early online dateJun 2018
DOIs
Publication statusPublished - 01 Nov 2018

Citation

Zhao, H.-M., Hu, R.-W., Chen, X.-X., Chen, X.-B., Lü, H., Li, Y.-W., . . . Wong, M.-H. (2018). Biodegradation pathway of di-(2-ethylhexyl) phthalate by a novel Rhodococcus pyridinivorans XB and its bioaugmentation for remediation of DEHP contaminated soil. Science of The Total Environment, 640-641, 1121-1131. doi: 10.1016/j.scitotenv.2018.05.334

Keywords

  • Phthalic acid esters
  • Biodegradation
  • Rhodococcus pyridinivorans
  • Phthalate dioxygenase gene
  • Biodegradation pathway
  • Bioremediation

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