Biodegradation of di-butyl phthalate (DBP) by a novel endophytic bacterium Bacillus subtilis and its bioaugmentation for removing DBP from vegetation slurry

Yu-Hong HUANG, Xue-Jing HUANG, Xiao-Hong CHEN, Quan-Ying CAI, Shaohua CHEN, Ce-Hui MO, Huixiong LÜ, Ming Hung WONG

Research output: Contribution to journalArticles

12 Citations (Scopus)

Abstract

Di-butyl phthalate (DBP) is a widely used plasticizer, recalcitrant and hazardous organic compound with high detection frequencies and concentrations in water and soil that pose a great threat to human health. A novel endphytic bacterium strain N-1 capable of efficiently degrading DBP and utilizing it as sole carbon source was isolated from Ageratum conyzoides. This bacterium was identified as Bacillus subtilis based on its morphological characteristics and 16S rDNA sequence analysis. Under the optimal culture conditions (pH 7.0, 30 °C), degradation percentage of DBP (12.5–100 mg/L) was up to 95% within five days, and its biodegradation half-life was less than 7.23 h. Degradation percentage of high DBP concentration (200 mg/L) was relatively lower (89%) with half-life of 56.8 h. DBP was degraded by Bacillus subtilis N-1 into mono-butyl phthalate and phthalic acid as evidenced by GC-MS analysis. Bioaugmentation of Youngia japonica plant slurry with strain N-1 greatly accelerated DBP dissipation with 97.5% removal percentage (higher by 47% than non-inoculation). The results highlighted that strain N-1 has great potential for bioremediation by plant-endophyte partnerships and for lowering PAE accumulation in crops. Copyright © 2018 Elsevier Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)1-9
JournalJournal of Environmental Management
Volume224
Early online dateJul 2018
DOIs
Publication statusPublished - Oct 2018

Citation

Huang, Y.-H., Huang, X.-J., Chen, X.-H., Cai, Q.-Y., Chen, S., Mo, C.-H., . . . Wong, M.-H. (2018). Biodegradation of di-butyl phthalate (DBP) by a novel endophytic bacterium Bacillus subtilis and its bioaugmentation for removing DBP from vegetation slurry. Journal of Environmental Management, 224, 1-9. doi: 10.1016/j.jenvman.2018.07.023

Keywords

  • Phthalic acid esters
  • Endophytes
  • Biodegradation
  • Degradation pathway
  • Bioaugmentation

Fingerprint Dive into the research topics of 'Biodegradation of di-butyl phthalate (DBP) by a novel endophytic bacterium Bacillus subtilis and its bioaugmentation for removing DBP from vegetation slurry'. Together they form a unique fingerprint.