Bio-plastics pha production by recombinant bacillus subtilis

Yujie WANG, Fenglin HUA, Yiu Fai TSANG, Shing Yan CHAN, Hong CHUA, Peter H.F. AU

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Polyhydroxyalkanoates (PHAs) are polyesters of hydroxyalkanoates synthesized by numerous bacteria as intracellular carbon and energy storage compounds and accumulated as granules in the cytoplasm of cells. In this work, we constructed two recombinant plasmids, pBE2Cl and pBE2C1AB. The two plasmids were inserted into Bacillus subtilis DB 104 and generated Bacillus subtilisl pBE2C1 and Bacillus subtilis/pBE2C1AB. The two recombinant strains were subjected to fermentation and showed PHA accumulation, the first reported example of mcl-PHA production in Bacillus subtilis. GC analysis identified the compound produced by Bacillus subtilis/ pBE2C1 was a hydroxydecanoate -cohydroxydodecanoate (HD-co-HDD) polymer while that produced by Bacillus subtilis/pBE2C1AB was a hydroxybutyrate-co-hydroxydecanoate-co-hydroxydodeca noate (HB-HD-HDD) polymer. The results also showed that the recombinant B. subtilis could utilize the malt waste in the medium as a carbon source better than that of glucose and thus could substantially lowered the cost of production of PHA. Copyright © 2007 Acta Press.

Original languageEnglish
Title of host publicationProceedings of the Second IASTED International Conference on Advanced Technology in the Environmental Field, ATEF 2006
EditorsL. UBERTINI
Place of PublicationCanada
PublisherACTA Press
Pages32-36
ISBN (Print)9780889865549
Publication statusPublished - 2007

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Bacilli
Polyhydroxyalkanoates
Plastics
Polymers
Plasmids
Carbon
Hydroxybutyrates
Polyesters
Fermentation
Energy storage
Bacteria
Glucose
Costs

Citation

Wang, Y. J., Hua, F. L., Tsang, Y. F., Chan, S. Y., Chua, H., & Yu, P. H. F. (2007). Bio-plastics pha production by recombinant bacillus subtilis. In L. Ubertini (Ed.), Proceedings of the Second IASTED International Conference on Advanced Technology in the Environmental Field, ATEF 2006 (pp. 32-36). Canada: Acta Press.

Keywords

  • Bacillus subtilis
  • Cloning and expression
  • PHA synthase gene
  • P (HB-co-mclHA)
  • Polyhydroxyalkanoates (PHAs)