Binary exposure to hypoxia and perfluorobutane sulfonate disturbs sensory perception and chromatin topography in marine medaka embryos

Lizhu TANG, Mengyuan LIU, Chenyan HU, Bingsheng ZHOU, Paul K.S. LAM, Chung Wah James LAM, Lianguo CHEN

Research output: Contribution to journalArticles

Abstract

Perfluorobutane sulfonate (PFBS), an environmental pollutant of emerging concern, is previously shown to dynamically interact with hypoxia on aquatic developmental toxicities. However, the molecular mechanisms underlying the interaction remain unknown. In this follow-up study, marine medaka embryos were exposed to 0 and 3.3 mg/L of PFBS under normoxia (6.9 mg/L) or hypoxia (1.7 mg/L) condition till 15 days post-fertilization. High-throughput transcriptomic sequencing was employed to filter differentially expressed genes and provide mechanistic insight into interactive action between hypoxia and PFBS. The results showed that hypoxia alone and the coexposure paradigm were similarly potent to modify transcriptional profiles, with the majority of genes significantly down-regulated. In contrast, transcriptional toxicity of PFBS was relatively milder. Functional annotation analyses found that hypoxia and coexposure groups mainly impacted phototransduction signaling by decreasing the transcriptions of cyclic nucleotide-gated (CNG) cation channels and retinol transport genes. However, this study demonstrated the first toxicological evidence that toxic effects of PFBS targeted the perception of chemical stimulus through olfactory and gustatory receptors. The addition of PFBS moderately exacerbated the toxic actions of hypoxia, which largely shaped the transcriptional pattern of coexposure group. In addition, gene interactive networks were constructed for hypoxia and coexposure groups, underlining the increased chromatin deacetylation and methylation to epigenetically repress genome-wide transcriptional initiation. Overall, PFBS and hypoxia interact to interrupt the embryonic development of sensory systems, which may compromise the individual fitness and survival, especially during early life stages when precocious perception of food and escape from predators are essential. Copyright © 2020 Elsevier Ltd. All rights reserved.
Original languageEnglish
Article number115284
JournalEnvironmental Pollution
Volume266
Issue numberPart 3
Early online dateAug 2020
DOIs
Publication statusE-pub ahead of print - Aug 2020

Citation

Tang, L., Liu, M., Hu, C., Zhou, B., Lam, P. K. S., Lam, J. C. W., & Chen, L. (2020). Binary exposure to hypoxia and perfluorobutane sulfonate disturbs sensory perception and chromatin topography in marine medaka embryos. Environmental Pollution, 266(Part 3). Retrieved from https://doi.org/10.1016/j.envpol.2020.115284

Keywords

  • PFBS
  • Hypoxia
  • Transcriptome
  • Sensory system
  • DNA remodeling
  • Marine medaka embryo

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