Benzothiazole-substituted benzofuroquinolinium dyes as new fluorescent probes for G-quadruplex DNA

Yu-Jing LU, Zheng-Ya WANG, Dong-Ping HU, Qiang DENG, Bao-Hua HUANG, Yan-Xiong FANG, Kun ZHANG, Wing Leung WONG, Cheuk Fai Stephen CHOW

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A series of new benzothiazole-benzofuroquinoline conjugates were designed and synthesized to enhance the performance for the application as a G-quadruplex fluorescent probe. All compounds were characterized and the selectivity, sensitivity, and stability of these dyes interacting with G-quadruplex were studied by using fluorescence titration, native PAGE experiments, FRET and CD analysis. Significant enhancement of fluorescent quantum yield was ob served when the compound bound with HRAS G-quadruplex. The results indicate that these compounds show much stronger binding affinity and fluorescent response to G-quadruplex than our previously reported benzothiazole-benzofuroquinoline core scaffold (CYTO) due to the incorporation of a specific amino moiety. The application of these new compounds as a fluorescent agent for living cell imaging was also demonstrated. Copyright © 2015 Published by Elsevier Ltd.
Original languageEnglish
Pages (from-to)94-102
JournalDyes and Pigments
Volume122
Early online dateJun 2015
DOIs
Publication statusPublished - 2015

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Fluorescent Dyes
DNA
Coloring Agents
Dyes
Quantum yield
Titration
Scaffolds
Fluorescence
Cells
Imaging techniques
Experiments
benzothiazole

Bibliographical note

Lu, Y.-J., Wang, Z.-Y., Hu, D.-P., Deng, Q., Huang, B.-H., Fang, Y.-X., et al. (2015). Benzothiazole-substituted benzofuroquinolinium dyes as new fluorescent probes for G-quadruplex DNA, Dyes and Pigments, 122, 94-102.

Keywords

  • G-quadruplex DNA
  • Benzothiazole-substituted benzofuroquinolinium dyes
  • Fluorescent probes
  • Cell imaging