Attachment of Balanus amphitrite larvae to biofilms originating from contrasting environments

O. S. HUNG, V. THIYAGARAJAN, R. ZHANG, Shiu Sun Rudolf WU, P. Y. QIAN

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

This study examined the attachment response of Balanus amphitrite larvae to bacteria-dominated biofilms originating from 4 sites of varying environmental conditions in the intertidal region of subtropical Hong Kong waters and during 2 seasons (winter and summer), under both laboratory and field conditions. Using multiple fingerprinting techniques (terminal restriction fragment length polymorphism, denaturing gradient gel electrophoresis and fluorescence in situ hybridization), we observed differences in the bacterial community composition of biofilms originating from the 4 sites. These biofilm samples were used to study the linkage between spatial changes in bacterial communities of biofilms and larval choice at the time of attachment. It was hypothesized that cyprids can distinguish biofilms originating from habitats that support higher recruitment. Both laboratory and field multiple-choice bioassays demonstrated that cyprids preferred to attach on biofilms originating from habitats where recruitment, juvenile growth and survival were the highest, thereby accepting the hypothesis proposed. This study did not identify particular bacterial species or groups in biofilms that attract or inhibit larval attachment, but we could correlate site-specific variations in bacterial community composition with larval choice, whereas bacterial abundance in biofilms was less important in this regard. Overall, this study highlights the significance of site-specific variation in biofilms on larval recruitment and demonstrated the discriminative behavior of barnacle larvae to biofilms originating from contrasting environments in the intertidal region. Thus, attachment cues from biofilms may also play a significant role in generating spatial variation in larval recruitment. Copyright © 2007 Inter-Research.

Original languageEnglish
Pages (from-to)229-242
JournalMarine Ecology Progress Series
Volume333
DOIs
Publication statusPublished - 12 Mar 2007

Fingerprint

Balanus amphitrite
biofilm
larva
larvae
bacterial communities
community composition
habitat
denaturing gradient gel electrophoresis
Cirripedia
fluorescence in situ hybridization
habitats
subtropics
linkage (genetics)
spatial variation
bioassay
electrokinesis
restriction fragment length polymorphism
polymorphism
fluorescence
gel

Citation

Hung, O. S., Thiyagarajan, V., Zhang, R., Wu, R. S. S., & Qian, P. Y. (2007). Attachment of Balanus amphitrite larvae to biofilms originating from contrasting environments. Marine Ecology Progress Series, 333, 229-242. doi: 10.3354/meps333229

Keywords

  • Balanus amphitrite
  • Biofilms
  • Larval attachment
  • Bacterial community
  • Barnacle recruitment