Assessment of typical pollutants in waterborne by combining active biomonitoring and integrated biomarkers response

Xiuting HE, Xiangping NIE, Zhaohui WANG, Zhang CHENG, Kaibin LI, Guiying LI, Ming Hung WONG, Ximei LIANG, Martin T.K. TSUI

Research output: Contribution to journalArticles

28 Citations (Scopus)

Abstract

Organic pollutants, heavy metals and pharmaceuticals are continuously dispersed into the environment and have become a relevant environmental emerging concern. In this study, a situ assay to assess ecotoxicity of mixed pollutants was carried out in three typical sites with different priority contaminations in Guangzhou, China. Chemical analysis of organic pollutants, metals and quinolones in three exposure sites were determined by GC–ECD/MS, ICP-AES and HPLC, as well as, a combination of biomarkers including: ethoxyresorufin O-deethylase (EROD); aminopyrine N-demethylase (APND); erythromycin N-demethylase (ERND); glutathione S-transferase (GST); malondialdehyde (MDA); CYP1A; and P-glycoprotein (P-gp) mRNA expressions were evaluated in Mugilogobius abei. Results of chemical analysis in sediment samples revealed that the dominant chemicals were organic pollutants and heavy metals in Huadi River while quinolones in the pond. Bioassays indicated that differences among sites were in relation to some specific biomarkers. EROD and GST activities significantly increased after 72 h in situ exposure, but no difference was observed among the exposure sites. APND, ERND and MDA exhibited dissimilar change patterns for different priority pollutants. CYP1A and P-gp mRNA expressions were significantly induced at all exposure sites, whilst P-gp activity was typical for S2 with the highest levels of quinolones. The molecular biomarkers seemed to be more susceptible than enzyme activities. These assays confirmed the usefulness of applying a large array of various combined biomarkers at different levels, in assessing the toxic effects of mixed pollutants in a natural aquatic environment. Copyright © 2011 Elsevier Ltd. Published by Elsevier Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)1422-1431
JournalChemosphere
Volume84
Issue number10
DOIs
Publication statusPublished - Sep 2011

Citation

He, X., Nie, X., Wang, Z., Cheng, Z., Li, K., Li, G., . . . Tsui, M. T. K. (2011). Assessment of typical pollutants in waterborne by combining active biomonitoring and integrated biomarkers response. Chemosphere, 84(10), 1422-1431. doi: 10.1016/j.chemosphere.2011.04.054

Keywords

  • Ecotoxicology
  • Mugilogobius abei
  • P450 enzyme activities
  • Antioxidant enzyme
  • CYP1A gene
  • P-glycoprotein gene

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