Arsenite transporters expression in rice (Oryza sativa L.) associated with arbuscular mycorrhizal fungi (AMF) colonization under different levels of arsenite stress

Xunwen CHEN, Hui LI, Wai Fung CHAN, Chuan WU, Fuyong WU, Shengchun WU, Ming Hung WONG

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34 Citations (Scopus)

Abstract

As a silicon hyperaccumulator, lowland rice takes up higher levels of As than many other plants due to silicic acid and arsenite sharing the same transporters (Lsi1 and Lsi2). Glomus intraradices (AH01) was inoculated to rice under different arsenite concentrations (0, 2 and 8 μM) in order to investigate the interactions between arbuscular mycorrhizal fungus and rice on the accumulation of arsenite. The relative mRNA expressions of Lsi1 and Lsi2 resulted in a down-regulating trend in mycorrhizal plants. Under 2 μM arsenite treatments, Lsi1 and Lsi2 were significantly decreased, by 0.7-fold (P < 0.05) and 0.5-fold (P < 0.01), respectively, in mycorrhizal plants when compared with non-mycorrhizal plants. This led to the decrease of arsenite uptake per unit of root dry mass. No organic As species were detected in both roots and shoots. The As(III)/As(V) ratios indicated that mycorrhizal plants immobilized most of the arsenite proportion in the roots and prevented its translocation from the roots to the shoots. Copyright © 2012 Elsevier Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)1248-1254
JournalChemosphere
Volume89
Issue number10
DOIs
Publication statusPublished - Nov 2012

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arsenite
Fungi
rice
colonization
fungus
Silicon
Acids
shoot
Silicic Acid
fold
silicic acid
translocation
silicon
Messenger RNA
Oryza

Citation

Chen, X., Li, H., Chan, W. F., Wu, C., Wu, F., Wu, S., & Wong, M. H. (2012). Arsenite transporters expression in rice (Oryza sativa L.) associated with arbuscular mycorrhizal fungi (AMF) colonization under different levels of arsenite stress. Chemosphere, 89(10), 1248-1254. doi: 10.1016/j.chemosphere.2012.07.054

Keywords

  • Oryza sativa
  • Crop
  • Mycorrhizae
  • Arsenic accumulation
  • Aquaporin
  • Real-time PCR