Arsenic biotransformation genes and As transportation in soil-rice system affected by iron-oxidizing strain (Ochrobactrum sp.)

Shengguo XUE, Xuan HE, Xingxing JIANG, Weisong PAN, Wai Chin LI, Libing XIA, Chuan WU

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5 Citations (Scopus)

Abstract

Arsenic (As) biotransformation in soil affects As biogeochemical cycling and is associated with As accumulation in rice. After inoculation with 1% iron-oxidizing bacteria (FeOB) in paddy soil, As speciation, As biotransformation genes in soil, As/Fe in Fe plaques, and As accumulation in rice were characterized. Compared with the control, the available As concentrations in soils decreased while amorphous and poorly crystalline Fe–Al oxidized As and crystalline Fe–Al oxidized As fractions increased of F (FeOB) and RF (rice and FeOB) treatments. Fe concentrations increased and positively correlated with As concentrations in Fe plaques on the rice root surface (***P < 0.001). Compared with R (rice), Monomethyl As (MMA), dimethyl As (DMA), arsenate (As(V)), and arsenite (As(III)) concentrations in rice plants showed a downwards trend of RF treatment. The As concentration in grains was below the National Standard for Food Safety (GB 2762-2017). A total of 16 As biotransformation genes in rhizosphere soils of different treatments (CK, F, R and RF were quantified by high-throughput qPCR (HT-qPCR). Compared with the control, the As(V) reduction and As transport genes abundance in other treatments increased respectively by 54.54%–69.17% and 54.63%–73.71%; the As(III) oxidation and As (de) methylation genes did not change significantly; however, several As(III) oxidation genes (aoxA, aoxB, aoxS, and arsH) increased. These results revealed that FeOB could reduce, transport As, and maybe also oxidize As. In addition, As(III) oxidation gene (aoxC) in rhizosphere soil was more abundant than in non-rhizosphere soil. It indicated that radial oxygen loss (ROL) promoted As(III) oxidation in rhizosphere soils. The results provide evidence for As biotransformation by ROL and FeOB in soil-rice system. ROL affects As oxidation and immobilization, and FeOB affects As reduction, transportation and may also affect As oxidation. Copyright © 2022 Elsevier Ltd. All rights reserved.

Original languageEnglish
Article number120311
JournalEnvironmental Pollution
Volume314
Early online dateSept 2022
DOIs
Publication statusPublished - Dec 2022

Citation

Xue, S., He, X., Jiang, X., Pan, W., Li, W., Xia, L., & Wu, C. (2022). Arsenic biotransformation genes and As transportation in soil-rice system affected by iron-oxidizing strain (Ochrobactrum sp.). Environmental Pollution, 314. Retrieved from https://doi.org/10.1016/j.envpol.2022.120311

Keywords

  • Soil-rice system
  • Iron oxidizing strain
  • Ferrous iron oxidation
  • Arsenic biotransformation gene

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