Appraisal of lignocellusoic biomass degrading potential of three earthworm species using vermireactor mediated with spent mushroom substrate: Compost quality, crystallinity, and microbial community structural analysis

Jinnashri DEVI, Utsab DEB, Soma BARMAN, Subhasish DAS, Satya Sundar BHATTACHARYA, Yiu Fai TSANG, Jin-Hong LEE, Ki-Hyun KIM

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Spent mushroom substrate (SMS) is a recalcitrant lignocellulosic waste. Recycling of SMS through composting has been reported; however, the process is lengthy due to its complex biochemical composition. Although vermitechnology is known for its high efficiency, it has rarely been applied to recycle SMS. In this study, the qualitative value of vermicomposted SMS mediated by three earthworm species (i.e., Eisenia fetida, Eudrilus eugeniae, and Perionyx excavatus) was evaluated on the basis of nutrient availability, microbial activity, phospholipid fatty acid (PLFA) profiles, and seed germination assays. Degradation profiles of the lignocellulosic substrate in the vermireactors were assessed by monitoring the changes in crystallinity and distribution of functional groups using X-ray diffraction (XRD) and Fourier transform infrared spectroscopy, respectively. Total organic carbon decreased by 1.4-3.5 folds with approximately 2.1-2.4 folds increase in nitrogen and phosphorus availability in all vermibeds. Interestingly, pH declined in the Eisenia and Eudrilus systems but increased in the Perionyx-vermibeds. XRD-derived crystallinity index was reduced significantly by 1.37 folds in Perionyx-vermicompost with concurrent microbial enrichment. Further, profuse abundance of vital functional groups (C=O, NH, and OH) was clearly observed in the vermicompost with Perionyx followed by that with Eisenia. Moreover, PLFA illustrated significant variations in fatty acid distributions and microbial communities of the three vermicomposting systems. The seed germination assay showed that the germination index and relative root-shoot vigor of Perionyx-vermicompost treated seeds were 1.05-1.30 times greater than those of Eisenia and Eudrilus vermicompost treated ones. The results suggest that SMS degradability was affected by the growth of a healthy microbial community through vermicomposting. Copyright © 2019 Elsevier B.V. All rights reserved.
Original languageEnglish
Article number135215
JournalScience of the Total Environment
Volume716
Early online dateNov 2019
DOIs
Publication statusPublished - May 2020

Citation

Devi, J., Deb, U., Barman, S., Das, S., Bhattacharya, S. S., Tsang, Y. F., . . . Kim, K.-H. (2020). Appraisal of lignocellusoic biomass degrading potential of three earthworm species using vermireactor mediated with spent mushroom substrate: Compost quality, crystallinity, and microbial community structural analysis. Science of The Total Environment, 716. Retrieved from https://doi.org/10.1016/j.scitotenv.2019.135215

Keywords

  • Spent mushroom substrate (SMS)
  • Vermicomposting
  • XRD crystallinity
  • Microbial community shift

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