Apolipoprotein B 3' hypervariable repeat genotype: Association with plasma lipid concentration, coronary artery disease, and other restriction fragment polymorphisms

June Hsieh WU, Ming-Shyan CHERN, Sing Kai LO, Ming-Shien WEN, Jau-Tsuen KAO

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Abstract

Apolipoprotein B gene 3' variable number tandem repeat (VNTR) and related regions were amplified by PCR and analyzed by agarose gel electrophoresis. Eighteen VNTR alleles (VNTR25, 26, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 58, 60) and 45 genotypes were observed in 477 Taiwanese subjects. The VNTR35 allele and genotype VNTR35/35 were observed most frequently in this population. The polymorphism information content was 0.62. Some minor alleles, such as VNTR25 and 60, were found only in coronary artery disease (CAD) and stroke patients in our sampling, and no statistically significant difference was observed in VNTR allelic frequency between control and CAD or stroke patients. Significant differences in allelic distribution of some VNTR alleles were observed between our normal Taiwanese population and a Caucasian group studied by others. VNTR43-47 and AluI+ (coding Ala591) restriction fragment length polymorphism (RFLP) as well as VNTR49-60 and EcoRI- (coding Lys4154) RFLP were found to be highly coinherited. No apparent association between the VNTR genotype and plasma lipid concentration was observed; however, for the same genotype, the CAD and stroke patients frequently showed higher lipoprotein(a) and lower HDL cholesterol concentrations than the control group. Copyright © 1996 The American Association for Clinical Chemistry.
Original languageEnglish
Pages (from-to)927-932
JournalClinical Chemistry
Volume42
Issue number6
Publication statusPublished - 1996

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Minisatellite Repeats
Apolipoproteins B
Polymorphism
Coronary Artery Disease
Genotype
Lipids
Plasmas
Alleles
Stroke
Restriction Fragment Length Polymorphisms
Lipoprotein(a)
Agar Gel Electrophoresis
Electrophoresis
Sepharose
HDL Cholesterol
Population
Genes
Gels
Association reactions
Sampling

Citation

Wu, J. H., Chern, M.-S., Lo, S.-K., Wen, M.-S., & Kao, J.-T. (1996). Apolipoprotein B 3' hypervariable repeat genotype: Association with plasma lipid concentration, coronary artery disease, and other restriction fragment polymorphisms. Clinical Chemistry, 42(6), 927-932.