Analysis of phytochelatins and other thiol-containing compounds by RP-HPLC with monobromobimane precolumn derivatization

Qin SUN, Zhihong YE, Xiaorong WANG, Ming Hung WONG

Research output: Contribution to journalArticles

5 Citations (Scopus)

Abstract

In this article, a method for quantitative determination of phytochelatins (PC n being the classic example) and other thiol-containing compounds in mixed standard solution and plant tissues is presented. Thiols were converted to fluorescent derivatives by precolumn derivatization with monobromobimane. The results showed that PC n and other thiol-containing compounds in standard mixed solutions were rapidly separated within 15 min by using a ACN 0.1% trifluoroacetic acid binary gradient elution. Glutathione was representatively selected to test the precision of this method. The calibration curve was linear in the range of 1.25–160 ng μl⁻¹ (regression coefficient r²=0.9999). It was confirmed that this method was rapid, simple, highly sensitive, stable, and had the property of simultaneous determination of PC n and other thiol-containing compounds. This method was applied to determine PC n and other thiol-containing compounds in a Cd hyperaccumulator Sedum alfredii in response to Cd. It was found that no PC n was detected in any tissue at any Cd treatment, suggesting that Cd hyperaccumulation and detoxification in this plant is not based on PC synthesis. Copyright © 2006 Higher Education Press and Springer-Verlag.
Original languageEnglish
Pages (from-to)54-58
JournalFrontiers of Chemistry in China
Volume1
Issue number1
DOIs
Publication statusPublished - Jan 2006

Citation

Sun, Q., Ye, Z., Wang, X., & Wong, M. (2006). Analysis of phytochelatins and other thiol-containing compounds by RP-HPLC with monobromobimane precolumn derivatization. Frontiers of Chemistry in China, 1(1), 54-58. doi: 10.1007/s11458-005-0010-9

Keywords

  • Phytochelatins
  • Thiol-containing compounds
  • RP-HPLC
  • Precolumn derivatization
  • Monobromobimane
  • Cd hyperaccumulator
  • Sedum alfredii

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