Abstract
Lipid derivatization technology-mediated fatty acid profiling studies have been suggested to dissect the contents of lipids in white fat and brown fat tissue. The focus of this study is to profile fatty acid lipidomics in brown adipose tissue and white adipose tissue of mice by derivatizing their lipids into fatty acid methyl esters via in situ transmethylation using a rice husk-derived biochar as porous media. The in situ transmethylation using biochar is advantageous in biological analysis because there was no loss of samples inevitably occurring in the loss of lipid in solvent extraction and purification steps. Copyright © 2017 Springer Science+Business Media Dordrecht.
Original language | English |
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Pages (from-to) | 1723-1728 |
Journal | Environmental Geochemistry and Health |
Volume | 41 |
Issue number | 4 |
Early online date | Apr 2017 |
DOIs | |
Publication status | Published - Aug 2019 |
Citation
Lee, J., Tsang, Y. F., Oh, J.-I., Hong, S., Kim, C., & Kwon, E. E. (2019). Analysis of fatty acids in mouse tissue via in situ transmethylation with biochar. Environmental Geochemistry and Health, 41(4), 1723-1728. doi: 10.1007/s10653-017-9965-yKeywords
- Lipid profiling
- Transmethylation
- Derivatization
- Tissue
- Biochar application