Analysis of fatty acids in mouse tissue via in situ transmethylation with biochar

Jechan LEE, Yiu Fai TSANG, Jeong-Ik OH, Seokmann HONG, Changsung KIM, Eilhann E. KWON

Research output: Contribution to journalArticle

Abstract

Lipid derivatization technology-mediated fatty acid profiling studies have been suggested to dissect the contents of lipids in white fat and brown fat tissue. The focus of this study is to profile fatty acid lipidomics in brown adipose tissue and white adipose tissue of mice by derivatizing their lipids into fatty acid methyl esters via in situ transmethylation using a rice husk-derived biochar as porous media. The in situ transmethylation using biochar is advantageous in biological analysis because there was no loss of samples inevitably occurring in the loss of lipid in solvent extraction and purification steps. Copyright © 2017 Springer Science+Business Media Dordrecht.
Original languageEnglish
Pages (from-to)1723–1728
JournalEnvironmental Geochemistry and Health
Volume41
Issue number4
Early online dateApr 2017
DOIs
Publication statusPublished - Aug 2019

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Fatty acids
Lipids
Fatty Acids
fatty acid
lipid
Tissue
Oils and fats
fat
Fats
biological analysis
Solvent extraction
ester
Purification
purification
Porous materials
porous medium
Esters
rice
tissue
in situ

Bibliographical note

Lee, J., Tsang, Y. F., Oh, J.-I., Hong, S., Kim, C., & Kwon, E. E. (2019). Analysis of fatty acids in mouse tissue via in situ transmethylation with biochar. Environmental Geochemistry and Health, 41(4), 1723–1728. doi: 10.1007/s10653-017-9965-y

Keywords

  • Lipid profiling
  • Transmethylation
  • Derivatization
  • Tissue
  • Biochar application