Airborne antibiotic resistance genes in Hong Kong kindergartens

Na LI, Yemao CHAI, Guang-Guo YING, Kevin C. JONES, Wenjing DENG

Research output: Contribution to journalArticle

Abstract

Antibiotic resistance genes (ARGs) and antibiotic resistant bacteria (ARB) have become a critical global public health issue in this century. There is increasing evidence for the presence and transmission of ARGs by air transmission. In this research, ARGs and ARB in air conditioner filter dust (AC dust) and urine samples from 55 kindergarten children in 17 kindergartens and nearby 10 soil samples in Hong Kong were analyzed. The results showed the presence of 16 ARG subtypes and the mobile genetic element (MGE) intI1 in AC dust, and 12 ARG subtypes in the soil samples. ARGs presenting resistance to sulfonamide (6.9 × 10⁻³–0.17) (expressed as relative abundance of the 16 S rRNA genes) were most abundant followed by macrolides (1.8 × 10⁻³–3.3 × 10⁻²), sul1, sul2 (sulfonamide), ermF (macrolides) and intI1 genes in AC dust in 17 kindergartens. For soil samples, 12 ARG subtypes and the intI1 were detected, and the genes providing resistance to sulfonamide (1.6 × 10⁻³–2.7 × 10⁻¹) were the most abundant ARGs in the 10 soil samples, followed by tetracycline (ND–1.4 × 10⁻²). Multi-resistant bacteria with sul1, sul2, intI1, or tetQ were detected in all AC dust samples and some urine samples. Based on bacterial genera and ARG co-occurrence network analysis and Hong Kong's special geographical location and cultural environment, there might be two origins for the ARGs detected in the kindergartens: β-lactam/macrolide ARGs mainly derived from human medicine use and tetracycline/sulfonamide ARGs mainly from other areas, as well as IntI1 may play a role in the spread of ARGs in Hong Kong. The widely detection of ARGs in AC dust in kindergartens in Hong Kong highlights the need for the improvement of management measures. Copyright © 2020 Elsevier Ltd. All rights reserved.
Original languageEnglish
Article number114009
JournalEnvironmental Pollution
Volume260
Early online dateJan 2020
DOIs
Publication statusE-pub ahead of print - Jan 2020

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Hong Kong
Antibiotics
Microbial Drug Resistance
Genes
Anti-Bacterial Agents
Air Filters
Dust
Sulfonamides
Macrolides
Soil
Air
Soils
Bacteria
Tetracycline
Urine
Bacterial Drug Resistance
Interspersed Repetitive Sequences
Lactams
Public health
rRNA Genes

Citation

Li, N., Chai, Y., Ying, G.-G., Jones, K. C., & Deng, W.-J. (2020). Airborne antibiotic resistance genes in Hong Kong kindergartens. Environmental Pollution, 260. Retrieved from https://doi.org/10.1016/j.envpol.2020.114009

Keywords

  • Indoor air pollution
  • Multi-resistant bacteria
  • Network analysis
  • Urine
  • Soil