A set of microsatellite primers for Zostera japonica (Zosteraceae)

Kai JIANG, Hui GAO, Na Na XU, Po Keung Eric TSANG, Xiao Yong CHEN

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Abstract

Premise of the study: Polymorphic microsatellite primers were developed in the seagrass Zostera japonica to investigate genetic variation and to identify clonal structure. Methods and Results: Thirteen polymorphic loci and 23 monomorphic loci were developed in Z. japonica. Two to 13 alleles per locus were observed at the polymorphic loci across 57 individuals of two Z. japonica populations. The observed and expected heterozygosities within populations ranged from 0.0000 to 1.0000 and from 0.0000 to 0.8542, respectively. Conclusions: Our study showed high-level polymorphism at the polymorphic loci in Z. japonica. These primers would be a powerful tool to study genetic variation, clonal structure, and mating systems. Copyright © 2011 Botanical Society of America.
Original languageEnglish
Pages (from-to)236-238
JournalAmerican Journal of Botany
Volume98
Issue number9
DOIs
Publication statusPublished - Sep 2011

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Zosteraceae
Zostera
Microsatellite Repeats
genetic variation
microsatellite repeats
loci
seagrass
reproductive strategy
heterozygosity
Population
allele
polymorphism
Alleles
mating systems
genetic polymorphism
alleles

Citation

Jiang, K., Gao, H., Xu, N.-N., Tsang, E. P. K., & Chen, X.-Y. (2011). A set of microsatellite primers for Zostera japonica (Zosteraceae). American Journal of Botany, 98(9), 236-238.

Keywords

  • Microsatellite primers
  • Polymorphism
  • Seagrass
  • Zostera japonica