A new bisphenol A derivative for estrogen receptor binding studies with surface plasmon resonance

Wing Leung WONG, Cheuk Fai Stephen CHOW

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A new bisphenol A (BPA) derivative was synthesized and immobilized onto the surface of a CM5 sensor chip for a binding study with orphan estrogen-related receptor γ using the surface plasmon resonance technique. The kinetic parameters, including dissociation and association constants and dissociation and association rate constants, were investigated. Moreover, a competitive inhibition assay was conducted to understand the competition relationship between the free BPA molecules in solution and the immobilized BPA molecules on the sensor chip surface. The CM5 sensor chip immobilized with the BPA derivative can be regenerated by using a high-concentration running buffer for repeated use more than 150 times without any adverse effect on its performance in the binding studies. The results indicate that the system has potential for further development as a sensitive surface plasmon resonance–based biosensor for endocrine-disrupting chemicals. Copyright © 2015 SETAC.
Original languageEnglish
Pages (from-to)1390-1396
JournalEnvironmental Toxicology and Chemistry
Volume34
Issue number6
Early online dateFeb 2015
DOIs
Publication statusPublished - 2015

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Surface Plasmon Resonance
Surface plasmon resonance
Estrogen Receptors
Derivatives
sensor
Sensors
Association reactions
Endocrine Disruptors
orphan
Molecules
Biosensing Techniques
Kinetic parameters
Biosensors
Rate constants
Assays
Buffers
Estrogens
assay
kinetics
bisphenol A

Citation

Wong, W.-L., & Chow, C.-F. (2015). A new bisphenol A derivative for estrogen receptor binding studies with surface plasmon resonance. Environmental Toxicology and Chemistry, 34(6), 1390-1396.

Keywords

  • Bisphenol A
  • Estrogen-related receptor
  • CM5 sensor chip
  • Surface plasmon resonance
  • Biosensor