A molecular fluorescent dye for specific staining and imaging of RNA in live cells: A novel ligand integration from classical thiazole orange and styryl compounds

Yu-Jing LU, Qiang DENG, Dong-Ping HU, Zheng-Ya WANG, Bao-Hua HUANG, Zhi-Yun DU, Yan-Xiong FANG, Wing Leung WONG, Kun ZHANG, Cheuk Fai Stephen CHOW

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35 Citations (Scopus)

Abstract

A new RNA-selective fluorescent dye integrated with a thiazole orange and a p-(methylthio)styryl moiety shows better nucleolus RNA staining and imaging performance in live cells than the commercial stains. It also exhibits excellent photostability, cell tolerance, and counterstain compatibility with 4′,6-diamidino-2-phenylindole for specific RNA–DNA colocalization in bioassays. Copyright © 2015 The Royal Society of Chemistry.
Original languageEnglish
Pages (from-to)15241-15244
JournalChemical Communications
Volume51
Issue number83
Early online dateAug 2015
DOIs
Publication statusPublished - 2015

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RNA
Fluorescent Dyes
Dyes
Ligands
Imaging techniques
Bioassay
Coloring Agents
thiazole orange
2-phenylindole

Citation

Lu, Y.-J., Deng, Q., Hu, D.-P., Wang, Z.-Y., Huang, B.-H., Du, Z.-Y., et al. (2015). A molecular fluorescent dye for specific staining and imaging of RNA in live cells: A novel ligand integration from classical thiazole orange and styryl compounds. Chemical Communications, 51(83), 15241-15244.