頭孢拉定降解菌的馴化、篩選及降解性能

從抗生素生產廠污水排放口取得菌種，並在實驗室中馴化、篩選出能以頭孢拉定為唯一碳源生長的好氧菌群，經過平板分離純化得到3株菌種。進行了各菌種以0、5、10、15、20、25 mg/L頭孢拉定為唯一碳源的4 d生長情況試驗，同時進行了10 mg/L頭孢拉定中生長4 d的菌種生長及降解初步試驗。結果表明，經過12代的馴化，篩選出的好氧混合菌群CDB (Cefradine Degradable Bacteria)，在底物質量濃度為0～25 mg/L時，4 d內均有最佳的生長和降解能力，而分離純化得到的單株菌中，CDB3菌株的生長情況及其降解能力均較強。各菌種的生長情況與其降解能力具有對應關係。
An aerobic mixed culture strains CDB (Cefradine Degradable Bacteria) were obtained from the wastewater discharge port of a factory which produces Antibiotics. Using Cefradine as the sole carbon source to isolate and culture 3 pure single strains. The 4 d degradation test in 0, 5, 10, 15, 20, 25 mg•L^(-1) Cefradine were done respectively and the results showed that all of the bacterial densities reached the peak after 4 d and slowed down as cultured in 10^(-25) mg•L^(-1) Cefradine. Results also showed that in the test of the degradation ability in 10 mg Lt Cefradine, the density of the mixed culture strains CDB has the highest degradation ability than what of the single strains. In the 3 single strains, the CDB3 has higher bacterium density and higher degradation ability than that of the other 2 stains. Strain which grow well in the 10 mg•L^(-1) Cefradine has higher degradation ability than the others. Copyright © 2008 華南師範大學.