粒徑和包裹物對納米銀在海洋微藻中的毒性影響

黄俊, 衣俊, 強麗媛, 程金平

Research output: Contribution to journalArticlespeer-review

Abstract

納米銀由於其獨特的抗菌特性廣泛應用於工商業領域。通過研究3種不同粒徑和包裹物的納米銀對典型海洋微藻中肋骨條藻(Skeletonema costatum)的毒性效應,進一步豐富納米銀對藻類毒性效應及其作用機制的認識。結果表明3種納米銀對矽藻細胞生長都有不同程度的生長抑制效應,其毒性大小依次為10 nm-OA>10 nm-PVP>20 nm-PVP。在細胞毒性方面,相同粒徑的聚乙烯吡咯烷酮(PVP)包裹的納米銀比油胺包裹(OA)的納米銀毒性更小;而相同包裹物的10 nm和20 nm兩種尺寸的納米銀的毒性效應主要取決於其在水環境中的粒徑和濃度,在濃度小於500μg·L⁻¹時,大粒徑的納米銀毒性更大;在濃度≥500μg·L⁻¹時,小粒徑的納米銀毒性更大。在光合毒性方面,50μg·L⁻¹的10 nm-PVP顯著上調3Hfcp A的表達(P<0.05)和顯著下調D1的表達(P<0.05),10 nm-OA在濃度為500μg·L⁻¹顯著上調3Hfcp A的表達(P<0.05),而20nm-PVP處理組沒有顯著性變化,說明光合作用相關基因的表達對小粒徑PVP包裹的納米銀更敏感。本研究結果表明,納米銀對海洋微藻的毒性是由粒徑大小、包裹物類型、暴露介質以及暴露濃度等多方面因素共同決定的。納米銀的粒徑越小毒性越大,粒徑對納米銀的毒性效應影響很大;而包裹物通過影響納米銀在水介質中的粒徑大小間接影響納米銀的毒性效應。
Due to the unique antibacterial properties, silver nanoparticles (AgNPs) have been widely used in commercial applications. In this study, the toxicity of three kinds of AgNPs with different sizes and surface coatings to marine diatom Skeletonema costatum (S. costatum) was studied, which was one of the dominant species in estuarine and coastal areas. All three kinds of tested AgNPs inhibited the growth of exposed S. costatum under acute exposure condition, and the order of toxicity was 10nm-OA>10nm-PVP>20nm-PVP. Given the condition of similar particle size, oil amine surface coated AgNPs were more toxic than polyvinyl pyrrolidone (PVP) surface coated AgNPs in S. costatum in term of cytotoxicity. With the same surface coating, the toxicity of AgNPs in S. costatum was affected by its hydrodynamic diameter and exposure concentrations. When the concentration of AgNPs was less than 500 μg·L⁻¹, larger sized AgNPs showed greater toxicity; When the concentration was greater than or equal to 500 μg·L⁻¹, smaller AgNPs exhibited greater toxicity. At molecular level, 50 μg·L⁻¹ 10nm-PVP significantly upregulated expression level of 3HfcpA (P <0.05) and significantly downregulated expression level of D1 (P<0.05), and 500 μg·L⁻¹ 10nm-OA significantly upregulated 3HfcpA expression (P<0.05), while 20nm-PVP treatment group didn't show any significant change. Exposed diatom demonstrated sensitive photosynthesis response to small size and PVP coated silver nanoparticles at molecular level. This study suggested that the toxicity of AgNPs to marine microalgae was largely controlled by the particle size, surface coating, exposure medium, exposure concentration and other factors. The smaller the particle size, the greater the toxicity of AgNPs, and the particle size of AgNPs played an important role in the toxicity of AgNPs in marine diatom S. costatum. Copyright © 2016 中國科學院生態環境研究中心.
Original languageChinese (Simplified)
Pages (from-to)1968-1977
Journal環境科學
Volume37
Issue number5
DOIs
Publication statusPublished - 15 May 2016

Citation

黃俊、衣俊、強麗媛和程金平(2016):粒徑和包裹物對納米銀在海洋微藻中的毒性影響,《環境科學》,37(5),頁1968-1977。

Keywords

  • 納米銀
  • 海洋微藻
  • 粒徑效應
  • 包裹物效應
  • 毒性比較
  • Marine microalgae
  • Particle size effects
  • Silver nanoparticles
  • Surface coating effects
  • Toxicity comparison
  • Alt. title: Contribution of particle size and surface coating of silver nanoparticles to its toxicity in marine diatom Skeletonema costatum