內分泌干擾物17β-雌二醇螢光分子印跡識別方法

劉峻, 林漢華, 隋洪艷, 周卓輝, 于紅霞

Research output: Contribution to journalArticles

Abstract

17β-雌二醇是近年來人們最為關注的重要內源性內分泌干擾物,它對生態系統和人體健康會產生一定的危害。採用分子印跡技術,通過丹磺醯氯與17β-雌二醇反應,合成出17β-雌二醇熒光標記物;建立了微球法合成17β-雌二醇熒光標記化合物分子印跡微球聚合物的方法,所獲得的微球粒徑均勻,平均為300~400 nm;分子印跡微球聚合物在不同極性溶劑中對17β-雌二醇熒光標記化合物的結合常數順序為乙腈>甲醇>水>二氯甲烷,其結合量的差異並不顯著,平均值為9.74μmol/g。該分子印跡微球聚合物可用於對復雜介質中17β-雌二醇的快速、簡便檢測。 Determination of 17β-estradiol in body fluids has been receiving much attention in recent years because the level of 17β-estradiol which has an effect on the endocrine systems in ecosystem and human health is increasing. Labellized by dansyl chloride, 17β-estradiol has been synthesized into fluorescent analyte successfully.In a microsphere way, the fluorescent 17β-estradiol can be turned into the molecularly imprinted polymer(MIP) which has the even size. The diameter of synthesized microsphere is between 300 to 400 nm. The order of the binding constant of microsphere MIP towards the fluorescent analyte in different polar solvent is acetonitrile > methanol > water > dichloromethane and the difference of binding site with an average of 9.74(μmol/g) is not significant. The research gives us the foundation for detecting the 17β-estradiol rapidly and conveniently in a complex medium. Copyright © 2006 中國環境科學研究院.
Original languageChinese
Pages (from-to)91-95
Journal環境科學研究
Volume2006
Issue number5
DOIs
Publication statusPublished - Nov 2006

Citation

劉峻、林漢華、隋洪艷、周卓輝和于紅霞(2006):內分泌干擾物17β-雌二醇熒光分子印跡識別方法,《環境科學研究》,2006(5),頁91-95。

Keywords

  • 17β-雌二醇
  • 分子印跡聚合物
  • 微球
  • 熒光分析物
  • 丹磺酰氯
  • 17β-estradiol
  • Molecularly imprinted polymer(MIP)
  • Microsphere
  • Fluorescent analyte
  • Dansyl chloride
  • Alt. title: The research of fluorescent sensing to endocrine disruptor 17β-estradiol measured by microsphere molecularly imprinted polymer